Lateral Flow Assay

Learn About Lateral Flow Assay

An immunoassay is a laboratory test that tests for the presence or absence of a particular antibody using the sample of blood as a positive control. The immunoassay consists of a single dose of antibody to be tested, either intravenously or intramuscularly, and the test is run under the condition that the antibody will be present in the sample of blood being tested. This allows for direct comparisons between samples from the same individual in order to determine whether the antibody is present in any samples of blood being tested.


The time required for an immunoassay depends on the type of antibody used and depends on the method used in performing the immunoassay. The time taken can range from about 10 minutes to about three hours depending on the antibody used. The time taken depends primarily on the size of the membrane used in the immunoassay (smaller cells can be tested more quickly but are usually less sensitive), and on the time required to draw the sample of blood. The two most common assays used are known as ‪sandwich> and ‪competitive‿.


Sandwiched assays are typically performed with a strip of antigen in the centre of a plate and an antibody on top of it. In this configuration, the sample of blood to be tested is applied onto the plate in the same way as it would be if a standard antigen was to be placed there. The antibody reacts with the antigen and the test will then provide a positive or negative result depending on whether the antibody reacts to the antigen. The advantage of a sandwich assay over other types is that a larger number of wells can be used for the test. However, sandwich assays are not as sensitive as other types. They are sometimes used to compare different levels of immunoreactivity between two groups. This means that the amount of antibody needed in order to detect the same antibody in two different samples may differ from one sample to another.


Competitive assays are performed by binding the sample of antibody with a known antigen. This allows for the detection of antibody binding to specific antigen rather than the whole antibody molecule. This also allows for more thorough testing of the amount of antibody needed to detect a specific antibody.


Both lateral flow assays and competitive assays are often used to determine the levels of antibodies needed to detect certain antibodies. These assays have been used in research for many years. Other uses of these methods include use in studies of the immune response to vaccinations such as those given during childhood. measles, mumps, and rubella vaccines.


In addition to the immune response to vaccines, the immunoassay can be used to determine the concentration of antibodies in the lymphatic fluid of patients who suffer from a variety of ailments. Because there is a greater concentration of antibodies in the lymphatic fluid than in the blood, this measurement is often used to monitor the response to treatment in patients with certain medical conditions.
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